Journal: iScience

Article Title: Reiterated male-to-female violence disrupts hippocampal estrogen receptor β expression, prompting anxiety-like behavior

doi: 10.1016/j.isci.2024.110585

Figure Lengend Snippet: Manipulation of ERβ signaling reproduces RMFVI-ignited behavioral perturbations and estradiol and BDNF decline (A and B) Elevated plus maze test for WT and ERβ KO, (A) percentage of time spent in the open arms from WT and ERβ KO mice, n = 8, p = 0.034 (unpaired t-test) (B) percentage of entries in the open arms from WT and ERβ KO mice, n = 8, p = 0.026 (unpaired t-test). (C) Survival Kaplan-Meir curves of WT and ERβ KO mice during RMFVI procedure, n = 9, p = 0.023. (D) Circulating 17-beta-estradiol measured by ELISA at baseline and after 21 days RMFVI in WT and ERβ KO mice, n = 5, baseline WT vs. baseline ERβ KO mice p = 0.033, WT baseline vs. 21 days RMFVI, p < 0.0001, ERβ KO mice baseline vs. 21 days RMFVI, p = 0.001 (Friedman's test). (E) Representative images of IF staining for BDNF in the DG (hilar cells), WT, and ERβ KO mice before and after RMFVI. (F) Quantification of IF staining for BDNF in the DG, control WTs vs. ERβ KO mice, before and after RMFVI, n = 5; WT vs. WT-RMFVI, p = 0.0004, WT vs. KO, p = 0.0002, WT vs. KO-RMFVI p < 0.0001 (Kruskal-Wallis one-way test). (G) Stereotaxic injection in the hippocampal formation: site of injection stained with trypan blue. (H) Light-dark box test, time spent by mice in the light compartment, n = 5; saline vs. PHTPP (antagonist), p = 0.013; Saline vs. DPN (agonist), p = 0.045 (Kruskal-Wallis one-way test). (I) Light-dark box test, transition to the light compartment, n = 5; saline vs. PHTPP (antagonist), p = 0.033 (Kruskal-Wallis one-way test).

Article Snippet: BDNF protein levels were determined by Enzyme-Linked Immune Assay (ELISA) (BDNF Immuno Assay System, Promega).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Control, Injection, Saline